We developed a novel high content assay to detect stimulated proliferation of primary human skeletal muscle satellite cells. This scatterplot shows the results for 1600 compounds screened, including all FDA-approved drugs and 500 natural products. We discovered that 15 compounds in this set were capable of stimulating satellite cells to induce proliferation. The cells used during this screen were obtained as a tissue sample of rectus abdominis from consensual donors.
In this screen, we found three very interesting natural classes of compounds; proliferators, toxic compounds and myogenic compounds. This becomes apparent when we plot proliferative status (cell number) versus cell size.
The most interesting compounds from this screen were Geraldol and Bromopride that consistently increased proliferation rates in satellite cells.