Validation and utilization of the biosensor using a fluorescent PPI reporter. (A) Glucose and exendin-4 stimulated insulin secretion. Inset shows relative fluorescent intensity of secreted mCherry-insulin. (B) Flow cytometry histogram for primary human b-cell sorting. LG, low glucose (5.6mM); HG, high glucose (25mM); Ex, exendin-4 10nM; NF, nifedipine 10mM; RFU, relative fluorescence units.
High Content assessment of insulin granularity using the INS-1 PPI-mCherry reporter system. When Bafilomycin is added, vesicle formation is inhibited and the insulin does not package into normal vesicles.